7V3W
Crystal Structure of VpsR display novel dimeric architecture and c-di-GMP binding: mechanistic implications in oligomerization, ATPase activity and DNA binding.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 |
Unit cell lengths | 118.788, 118.788, 79.146 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.505 - 3.205 |
R-factor | 0.2315 |
Rwork | 0.224 |
R-free | 0.27320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ny5 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.942 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.510 | 3.430 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.065 | 1.373 |
Rmeas | 0.077 | 1.651 |
Rpim | 0.042 | 0.894 |
Number of reflections | 20013 | 1896 |
<I/σ(I)> | 11.9 | |
Completeness [%] | 97.6 | 98.7 |
Redundancy | 2.9 | |
CC(1/2) | 0.997 | 0.363 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.8M AMS, bicine pH 8.8 NaCl, 300mM |