7V3E
The complex structure of soBcmB and its intermediate product 1a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 97.878, 180.533, 133.298 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.460 - 2.120 |
| R-factor | 0.218 |
| Rwork | 0.217 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v3o |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.803 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.740 | 2.230 |
| High resolution limit [Å] | 2.115 | 2.120 |
| Rmerge | 0.139 | 1.157 |
| Number of reflections | 67597 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.0M (NH4)2HPO4, 0.1M imidazole pH 8.0, 0.2M NaCl |
