7V36
The complex structure of soBcmB and its intermediate product 2a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 100.905, 100.905, 132.771 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.693 - 1.960 |
R-factor | 0.180069405563 |
Rwork | 0.179 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7v3o |
RMSD bond length | 0.007 |
RMSD bond angle | 0.879 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.693 | 2.010 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.103 | 1.090 |
Number of reflections | 28436 | 1990 |
<I/σ(I)> | 13.9 | |
Completeness [%] | 97.8 | |
Redundancy | 7.2 | |
CC(1/2) | 0.998 | 0.719 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Tris-HCl pH 8.0, 1.5M Ammonium sulfate |