7V2Z
ZIKV NS3helicase in complex with ssRNA and ATP-Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.620, 72.356, 59.507 |
| Unit cell angles | 90.00, 94.15, 90.00 |
Refinement procedure
| Resolution | 40.810 - 2.101 |
| R-factor | 0.191042529433 |
| Rwork | 0.189 |
| R-free | 0.23934 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jmt |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.877 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.890 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.054 | 0.443 |
| Number of reflections | 25785 | 1307 |
| <I/σ(I)> | 2.72 | |
| Completeness [%] | 97.6 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M potassium sodium tartrate tetrahydrate pH 7.4 and 20% (w/v) polyethylene glycol 3350 |
