7V2X
The complex structure of soBcmB and its substrate 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97852 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 101.097, 101.097, 130.377 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.130 - 2.084 |
| R-factor | 0.194042906047 |
| Rwork | 0.192 |
| R-free | 0.22854 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v2t |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.130 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.104 | 1.633 |
| Number of reflections | 23036 | 1769 |
| <I/σ(I)> | 26.7 | |
| Completeness [%] | 94.9 | |
| Redundancy | 31.1 | |
| CC(1/2) | 1.000 | 0.863 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 7.0, 2 M Ammonium sulfate |
