7V2V
Crystal Structure of VpsR display novel dimeric architecture and c-di-GMP binding: mechanistic implications in oligomerization, ATPase activity and DNA binding.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9724 |
| Spacegroup name | P 31 |
| Unit cell lengths | 119.921, 119.921, 81.601 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.319 - 3.194 |
| R-factor | 0.2395 |
| Rwork | 0.234 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ny5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 3.310 |
| High resolution limit [Å] | 3.190 | 3.190 |
| Rmerge | 0.105 | 1.123 |
| Rmeas | 0.121 | 1.330 |
| Rpim | 0.059 | 0.697 |
| Number of reflections | 21513 | 2212 |
| <I/σ(I)> | 6.8 | 0.6 |
| Completeness [%] | 98.2 | 99.3 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.990 | 0.314 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | AMS bicine pH 8.8 300 NaCl |
