7V2S
Crystal structure of juvenile hormone acid methyltransferase JHAMT isoform3 from silkworm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.057, 52.564, 57.261 |
| Unit cell angles | 69.01, 75.73, 83.35 |
Refinement procedure
| Resolution | 52.137 - 2.133 |
| Rwork | 0.195 |
| R-free | 0.24170 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.140 | 2.220 |
| High resolution limit [Å] | 2.130 | 2.130 |
| Rmerge | 0.114 | 0.452 |
| Rmeas | 0.136 | |
| Number of reflections | 28167 | 2312 |
| <I/σ(I)> | 17.11 | |
| Completeness [%] | 94.3 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 0.1M Tris pH8.5, 0.2M magnesium chloride, 20% polyethylene glycol 3350 |
