7V2G
The 0.98 angstrom structure of the human FABP3 Y19F mutant complexed with palmitic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.588, 69.726, 33.938 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.220 - 0.980 |
| R-factor | 0.10224 |
| Rwork | 0.101 |
| R-free | 0.11672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wvm |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.279 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.000 |
| High resolution limit [Å] | 0.980 | 0.980 |
| Rmerge | 0.093 | 0.393 |
| Number of reflections | 71003 | 3386 |
| <I/σ(I)> | 7.7 | 5.4 |
| Completeness [%] | 94.5 | |
| Redundancy | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl (pH 8.5), 50% PEG 400 |
