7V1X
Difructose dianhydride I synthase/hydrolase (alphaFFase1) from Bifidobacterium dentium in complex with beta-D-fructofuranose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.439, 156.304, 100.744 |
| Unit cell angles | 90.00, 110.09, 90.00 |
Refinement procedure
| Resolution | 48.690 - 1.760 |
| R-factor | 0.153 |
| Rwork | 0.151 |
| R-free | 0.18490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kq7 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.688 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.690 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.115 | 0.799 |
| Rmeas | 0.124 | 0.869 |
| Rpim | 0.047 | 0.337 |
| Number of reflections | 279590 | 13723 |
| <I/σ(I)> | 11.8 | 2.4 |
| Completeness [%] | 99.3 | |
| Redundancy | 7 | |
| CC(1/2) | 0.998 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20% PEG3350, 0.2 M di-sodium tartrate |
