7V1R
Leifsonia Alcohol Dehydrogenases LnADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.944, 237.485, 68.784 |
| Unit cell angles | 90.00, 113.03, 90.00 |
Refinement procedure
| Resolution | 49.490 - 2.810 |
| R-factor | 0.2486 |
| Rwork | 0.246 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7v1q |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.474 |
| Data scaling software | HKL-3000 |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.171 | 0.498 |
| Number of reflections | 38243 | 1927 |
| <I/σ(I)> | 11.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M Sodium acetate/Acetic acid, pH 7.0, 0.2M Zinc acetate, 20% PEG 1000 |
