7V1L

Structure of sNASP core in complex with H3 alpha3 helix peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9766
Spacegroup name	C 2 2 21
Unit cell lengths	66.902, 177.051, 65.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.742 - 2.849
R-factor	0.2031
Rwork	0.201
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nq0
RMSD bond length	0.008
RMSD bond angle	0.935
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.950
High resolution limit [Å]	2.849	6.130	2.850
Rmerge	0.142	0.059
Rmeas	0.149	0.062
Rpim	0.045	0.018	0.390
Number of reflections	9497	1030	926
<I/σ(I)>	2.9
Completeness [%]	99.8	99.9	98.4
Redundancy	12	12.1	10
CC(1/2)		0.998	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.4M Na-K phosphate, pH 8.2