7V1K
Apo structure of sNASP core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-01-07 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 62 2 2 |
Unit cell lengths | 95.358, 95.358, 206.229 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.178 - 3.287 |
R-factor | 0.2406 |
Rwork | 0.239 |
R-free | 0.26090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nq0 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.876 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.420 |
High resolution limit [Å] | 3.287 | 7.100 | 3.300 |
Rmerge | 0.145 | 0.046 | |
Rmeas | 0.147 | 0.046 | |
Rpim | 0.035 | 0.008 | 0.420 |
Number of reflections | 9058 | 1034 | 870 |
<I/σ(I)> | 1.9 | ||
Completeness [%] | 99.9 | 99.8 | 99.8 |
Redundancy | 33.2 | 31.8 | 25.9 |
CC(1/2) | 1.000 | 0.518 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Calcium acetate, 0.1M HEPES (pH7.5), 40% v/v PEG 400 |