7UZ2
Structure of beta-glycosidase from Sulfolobus solfataricus in complex with C5a-fluoro-valienide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 168.265, 168.265, 95.504 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.419 - 1.830 |
| Rwork | 0.207 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uwr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 1.880 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.027 | 1.115 |
| Number of reflections | 136568 | 6735 |
| <I/σ(I)> | 11.6 | 0.6 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 10.2 | |
| CC(1/2) | 1.000 | 0.280 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10 - 13 % PEG 4000, 0.1 M Sodium Acetate, 0.2M Ammonium Acetate, 5% ethylene glycol. |
