7UZ0
AntiT-tRNA flip UCCA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 46.168, 56.695, 268.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.610 - 3.030 |
| R-factor | 0.2478 |
| Rwork | 0.246 |
| R-free | 0.27670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mgn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.610 | 3.141 |
| High resolution limit [Å] | 3.030 | 3.030 |
| Rmerge | 0.131 | 1.135 |
| Rmeas | 0.141 | 1.220 |
| Rpim | 0.053 | 0.446 |
| Number of reflections | 13078 | 688 |
| <I/σ(I)> | 12.9 | 1.59 |
| Completeness [%] | 99.2 | 98.55 |
| Redundancy | 7.1 | 7.3 |
| CC(1/2) | 0.997 | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 10% MPD, 40 mM sodium cacodylate, pH 7, 12 mM spermine hydrochloride, 40 mM lithium chloride, 80 mM strontium chloride, 20 mM magnesium chloride |
