7UYY
The crystal structure of the Pseudomonas aeruginosa aldehyde dehydrogenase encoded by the PA4189 gene in complex with NADH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 184.518, 184.518, 251.136 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.920 - 2.700 |
| R-factor | 0.1374 |
| Rwork | 0.135 |
| R-free | 0.18130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6b4r |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.113 | 0.382 |
| Number of reflections | 44964 | 6493 |
| <I/σ(I)> | 20 | 7.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.17 M Lithium Sulfate monohydrate, 0.085 M Tris Hydrochloride pH 8.5, 26.6% w/v Polyethylene Glycol 4000, 15% v/v Glycerol |
