7UYK

Structure of RNF31 in complex with FP06655, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97932
Spacegroup name	C 1 2 1
Unit cell lengths	127.730, 70.140, 89.220
Unit cell angles	90.00, 134.18, 90.00

Refinement procedure

Resolution	44.610 - 2.700
R-factor	0.2001
Rwork	0.198
R-free	0.24550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dbg
RMSD bond length	0.003
RMSD bond angle	0.701
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.610	2.790
High resolution limit [Å]	2.700	2.700
Rmerge	0.070	0.810
Number of reflections	15585	1551
<I/σ(I)>	10.8
Completeness [%]	99.5
Redundancy	3.8
CC(1/2)	0.998	0.519

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	10% w/v glycerol, 20% w/v ethanol