7UYJ

Structure of RNF31 in complex with FP06652, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 31 1 2
Unit cell lengths	95.370, 95.370, 93.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	61.800 - 2.320
R-factor	0.2203
Rwork	0.218
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dbg
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.800	2.400
High resolution limit [Å]	2.320	2.320
Number of reflections	21131	2105
<I/σ(I)>	11.7
Completeness [%]	99.8
Redundancy	11.5
CC(1/2)	0.999	0.576

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Amino Acids (0.2M DL-Glutamic acid monohydrate; 0.2M DL-Alanine; 0.2M Glycine; 0.2M DL-Lysine monohydrochloride; 0.2M DL-Serine),0.1 M Buffer System 1 (Imidazole; MES monohydrate (acid) pH 6.5), 30.0% Precipitant Mix 1 (40% v/v PEG 500* MME; 20 % w/v PEG 20000)