7UY2

Structure of RNF31 in complex with FP06649, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-31
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9201
Spacegroup name	P 61
Unit cell lengths	49.420, 49.420, 267.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.800 - 2.510
R-factor	0.2142
Rwork	0.211
R-free	0.27010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dbg
RMSD bond length	0.002
RMSD bond angle	0.549
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.800	2.600
High resolution limit [Å]	2.510	2.510
Rmerge	0.192
Number of reflections	12531	1268
<I/σ(I)>	10
Completeness [%]	99.8
Redundancy	11.8
CC(1/2)	0.997	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.06 M Divalents (0.3M Magnesium chloride hexahydrate; 0.3M Calcium chloride dihydrate), 0.1 M Buffer System 1 (Imidazole; MES monohydrate (acid) pH 6.5), 37.5% Precipitant Mix 4 (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)