7UWO

Structure of beta-catenin in complex with FP05874, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-23
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	68.128, 85.780, 205.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.350 - 2.750
R-factor	0.2068
Rwork	0.204
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jdh
RMSD bond length	0.004
RMSD bond angle	0.651
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.350	2.848
High resolution limit [Å]	2.750	2.750
Rmerge	0.067	0.667
Number of reflections	15896	1555
<I/σ(I)>	16.64
Completeness [%]	98.5
Redundancy	6.7
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.05M MgCl2, 0.1M MES pH6.5, 5% (w/v) PEG 4000