7UWI

Structure of beta-catenin in complex with FP01567, a Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-24
Detector	RIGAKU SATURN 92
Wavelength(s)	0.97949
Spacegroup name	P 21 21 21
Unit cell lengths	49.680, 91.810, 113.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.340 - 2.320
R-factor	0.2378
Rwork	0.236
R-free	0.27750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qz7
RMSD bond length	0.003
RMSD bond angle	0.545
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20_4444)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.340	2.403
High resolution limit [Å]	2.320	2.320
Rmerge	0.115
Number of reflections	23082	2271
<I/σ(I)>	8.6
Completeness [%]	99.7
Redundancy	7.1
CC(1/2)	0.998	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	0.1 M Sodium phosphate monobasic monohydrate, 0.1 M Potassium phosphate monobasic, 0.1 M MES monohydrate pH 6.5, 2.0 M Sodium chloride