7UVC

Rad6(P43L)-Bre1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	77
Detector technology	PIXEL
Collection date	2019-01-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 2
Unit cell lengths	240.433, 50.088, 57.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.720 - 3.050
R-factor	0.2367
Rwork	0.234
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uv8
Data reduction software	XDS
Data scaling software	pointless
Phasing software	AMoRE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.260
High resolution limit [Å]	3.050	3.050
Number of reflections	13829	13829
<I/σ(I)>	11.5
Completeness [%]	99.9	99.9
Redundancy	9.7	9.4
CC(1/2)	0.999	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	286	0.05 to 0.1 M MMT buffer and 15-25% PEG400