7UV8
Rad6-Bre1 Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-09-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | .987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 241.580, 49.951, 57.568 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.490 - 2.700 |
| R-factor | 0.242 |
| Rwork | 0.240 |
| R-free | 0.28000 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.550 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 19967 | 710 |
| <I/σ(I)> | 20.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 37.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 286.15 | 0.05 to 0.1 M MMT buffer and 15-25% PEG400 |
