7UUM

Crystal structure of aminoglycoside resistance enzyme ApmA, complex with paromomycin and coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 3 2
Unit cell lengths	133.748, 133.748, 133.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.750 - 2.740
R-factor	0.2243
Rwork	0.223
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm2
RMSD bond length	0.003
RMSD bond angle	0.725
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.790
High resolution limit [Å]	2.740	2.740
Rmerge	0.197	1.791
Rpim	0.036	0.548
Number of reflections	11364	548
<I/σ(I)>	20.21	1.31
Completeness [%]	100.0	100
Redundancy	30.1	13.2
CC(1/2)	1.000	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M litihium sulfate, 0.1 M Tris pH 8.5, 23% PEG3350, 14 mM paromomycin