7UUL

Crystal structure of aminoglycoside resistance enzyme ApmA, complex with kanamycin B and coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2020-10-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	101.093, 131.685, 157.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.470 - 2.260
R-factor	0.1734
Rwork	0.172
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm2
RMSD bond length	0.012
RMSD bond angle	1.419
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.470	2.300
High resolution limit [Å]	2.260	2.260
Rmerge	0.085	0.888
Rpim	0.034	0.436
Number of reflections	99115	4910
<I/σ(I)>	22.2	1.3
Completeness [%]	99.4	99.4
Redundancy	6.9	4.8
CC(1/2)	0.999	0.889

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M litium sulfate, 0.1 M Tris pH 8.5, 25% PEG3350, 5 mM kanamycin B