7UUK

Crystal structure of aminoglycoside resistance enzyme ApmA, complex with tobramycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-06-29
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	62.146, 107.405, 134.976
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.850 - 2.820
R-factor	0.1994
Rwork	0.197
R-free	0.25420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7jm2
RMSD bond length	0.004
RMSD bond angle	0.660
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.870
High resolution limit [Å]	2.820	2.820
Rmerge	0.158	0.702
Rpim	0.078	0.389
Number of reflections	21504	1002
<I/σ(I)>	5.9	1.01
Completeness [%]	96.4	93.7
Redundancy	5	4.1
CC(1/2)	0.996	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	0.2 M calcium chloride, 20% PEG3350, 5 mM tobramycin