7UU3

Crystal structure of APOBEC3G complex with 3'overhangs RNA-Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03320
Spacegroup name	P 1
Unit cell lengths	60.709, 67.192, 79.624
Unit cell angles	98.29, 108.72, 112.66

Refinement procedure

Resolution	45.695 - 3.099
R-factor	0.1739
Rwork	0.170
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6p3x
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.210
High resolution limit [Å]	3.099	3.099
Number of reflections	61081	4248
<I/σ(I)>	8.89
Completeness [%]	98.2	93.7
Redundancy	3.3	2.4
CC(1/2)	0.990	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.3	291	0.1 M MES, pH 6.3, 12% PEG 20K