7UTE

MicroED structure of Aeropyrum pernix protoglobin mutant

Experimental procedure

Collection date	2022-03-29
Spacegroup name	P 1
Unit cell lengths	46.246, 58.305, 80.732
Unit cell angles	104.08, 98.59, 90.10

Refinement procedure

Resolution	24.260 - 2.100
R-factor	0.1983
Rwork	0.197
R-free	0.22090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model from AlphaFold
RMSD bond length	0.013
RMSD bond angle	1.558
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.391	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	34624	2481
<I/σ(I)>		2.4
Completeness [%]		71
CC(1/2)	0.972	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1