7USV

Plasmodium falciparum protein Pfs230 D1 in complex with nanobody F10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 21 21 21
Unit cell lengths	48.083, 101.221, 148.185
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.894 - 2.100
R-factor	0.1999
Rwork	0.198
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7usr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.894	47.894	2.220
High resolution limit [Å]	2.100	6.240	2.100
Rmerge	0.227	0.099	1.358
Rmeas	0.242	0.106	1.434
Total number of observations	422617
Number of reflections	43202	1806	6681
<I/σ(I)>	11.83	33.92	1.76
Completeness [%]	99.5	99.6	96.9
Redundancy	9.782	9.41	9.734
CC(1/2)	0.980	0.996	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	0.2 M ammonium sulphate, 0.1 M sodium acetate pH 4.6, and 25% PEG 4000