7UST

Plasmodium falciparum protein Pfs230 D1 in complex with nanobody F5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	C 2 2 21
Unit cell lengths	42.299, 84.179, 184.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.090 - 1.700
R-factor	0.1911
Rwork	0.189
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7usr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.220	46.220	1.800
High resolution limit [Å]	1.700	5.070	1.700
Rmerge	0.048	0.026	1.004
Rmeas	0.050	0.027	1.047
Total number of observations	445981
Number of reflections	36891	1527	5832
<I/σ(I)>	24.85	85.1	2.17
Completeness [%]	99.9	99.8	99.2
Redundancy	12.089	11.604	12.418
CC(1/2)	1.000	1.000	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281	2.0 M ammonium sulphate, 0.1 sodium citrate pH 5.5