7USS

Plasmodium falciparum protein Pfs230 Pro-D1D2 - Structure of the first two 6-cysteine domains with N-terminal extension

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953732
Spacegroup name	P 1
Unit cell lengths	35.250, 50.853, 108.965
Unit cell angles	99.19, 98.86, 94.66

Refinement procedure

Resolution	48.540 - 1.900
R-factor	0.1788
Rwork	0.177
R-free	0.20680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7usr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15_3459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.540	48.540	2.010
High resolution limit [Å]	1.900	5.660	1.900
Rmerge	0.087	0.034	0.738
Rmeas	0.102	0.040	0.872
Total number of observations	205972
Number of reflections	56540	2155	8901
<I/σ(I)>	9.44	31.63	1.57
Completeness [%]	97.3	98.6	94.9
Redundancy	3.643	3.655	3.495
CC(1/2)	0.997	0.998	0.685

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	30% PEG MME 2000, 0.1 M potassium thiocyanate