7USN

Crystal structure of ferritin 1 from Caenorhabditis elegans, FTN-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	P 2 3
Unit cell lengths	227.763, 227.763, 227.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.559 - 1.789
Rwork	0.166
R-free	0.18850
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.304
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELXD
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.789	1.790
Rmerge	0.162	1.567
Rpim	0.036	0.347
Number of reflections	365928	17721
<I/σ(I)>	12.2
Completeness [%]	99.9
Redundancy	21.1
CC(1/2)	0.999	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	Ammonium sulfate, Bis-Tris