7URY
Tetradecameric hub domain of CaMKII beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2021-07-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 105.712, 182.795, 110.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.230 - 2.640 |
| R-factor | 0.2054 |
| Rwork | 0.204 |
| R-free | 0.23530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7urw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.324 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.640 | 7.160 | 2.640 |
| Rmerge | 0.072 | 0.035 | 0.443 |
| Rmeas | 0.083 | 0.041 | 0.508 |
| Rpim | 0.040 | 0.020 | 0.241 |
| Total number of observations | 102591 | ||
| Number of reflections | 28300 | 1424 | 1422 |
| <I/σ(I)> | 4.4 | ||
| Completeness [%] | 88.9 | 82.7 | 91.5 |
| Redundancy | 3.6 | 3.9 | 3.7 |
| CC(1/2) | 0.997 | 0.826 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROFLUIDIC | 7 | 293 | 0.1 M imidazole pH 7, 0.15 M DL-Malic acid, 22% PEG methyl ether 550 |
