7URH

Crystal structure of Ferritin 2 from Caenorhabditis elegans, FTN-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	I 4
Unit cell lengths	154.840, 154.840, 128.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.505 - 1.468
Rwork	0.159
R-free	0.17640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wpn
RMSD bond length	0.004
RMSD bond angle	1.197
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.490
High resolution limit [Å]	1.468	1.470
Rmerge	0.081	0.625
Rpim	0.034	0.262
Number of reflections	256649	11916
<I/σ(I)>	9.4
Completeness [%]	99.7
Redundancy	6.8
CC(1/2)	0.998	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2-methyl-2,4-pentanediol, Bis-Tris, Ammonium acetate