7UQW
PCC6803 Cyanophycinase S132DAP covalently bound to cyanophycin dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-13 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.463, 132.947, 164.092 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.610 - 1.500 |
R-factor | 0.1821 |
Rwork | 0.181 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3en0 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.654 |
Data reduction software | XDS |
Data scaling software | pointless |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.610 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 133142 | 13155 |
<I/σ(I)> | 15.18 | 3.1 |
Completeness [%] | 99.9 | |
Redundancy | 12.9 | |
CC(1/2) | 0.997 | 0.866 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M MES pH 6.6, 1.65 M ammonium sulfate and 4% formamide |