7UQN

Pathogenesis related 10-10 noscapine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	48.871, 71.565, 91.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.940 - 1.700
R-factor	0.1964
Rwork	0.192
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uql
RMSD bond length	0.013
RMSD bond angle	1.507
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.940	1.750
High resolution limit [Å]	1.700	1.700
Number of reflections	17557	1021
<I/σ(I)>	37.17
Completeness [%]	96.8
Redundancy	12.4
CC(1/2)	1.000	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Bis-Tris pH 5.5