7UPZ

Structural basis for cell type specific DNA binding of C/EBPbeta: the case of cell cycle inhibitor p15INK4b promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	C 2 2 21
Unit cell lengths	101.699, 112.758, 75.528
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.181 - 2.487
R-factor	0.2151
Rwork	0.212
R-free	0.26410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gtw
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.487	2.487
Rmerge	0.056	1.160
Rpim	0.024	0.506
Number of reflections	15346	769
<I/σ(I)>	28.8	1.9
Completeness [%]	99.4
Redundancy	6.6
CC(1/2)	0.979	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	100 mM potassium chloride, 10 mM magnesium chloride, 50 mM MES, pH 6.0, 10% v/v PEG400, 5% v/v glycerol