7UPV

Structure of maize BZR1-type beta-amylase provides new insights into its noncatalytic adaptation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	50.896, 69.141, 59.123
Unit cell angles	90.00, 95.04, 90.00

Refinement procedure

Resolution	44.830 - 1.840
R-factor	0.2113
Rwork	0.208
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bya
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	35028	3198
<I/σ(I)>	14.7
Completeness [%]	98.2
Redundancy	3.2
CC(1/2)		0.187

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	0.2M MgCl2, 0.1M Sodium citrate tribasic dihydrate, 8% Polyethylene glycol 20000