7ULF
l-glutamate/GTP complex of F420-gamma glutamyl ligase (CofE) from Archaeoglobus fulgidus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-12 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.95370 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 68.710, 68.710, 92.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.040 - 1.610 |
R-factor | 0.1921 |
Rwork | 0.191 |
R-free | 0.21180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2phn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.409 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.040 | 1.640 |
High resolution limit [Å] | 1.610 | 1.610 |
Rpim | 0.035 | 0.645 |
Number of reflections | 29419 | 1435 |
<I/σ(I)> | 11.7 | 1.3 |
Completeness [%] | 100.0 | |
Redundancy | 7.1 | |
CC(1/2) | 0.998 | 0.544 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.8 M ammonium sulfate, 0.1 M citrate pH 4.5, 2 mM GTP, 5 mM Mn2+, 1 mM L-glutamate |