7UK8

Apo form of YgiC from Escherichia coli K-12

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-29
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9537
Spacegroup name	P 31 2 1
Unit cell lengths	88.220, 88.220, 141.598
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.200 - 1.850
R-factor	0.1868
Rwork	0.186
R-free	0.21110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7uk6
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.200	38.200	1.890
High resolution limit [Å]	1.850	9.070	1.850
Rmerge	0.095	0.062	2.735
Rmeas	0.097	0.063	2.805
Rpim	0.022	0.014	0.620
Total number of observations	1121209	10164	65461
Number of reflections	54990	543	3257
<I/σ(I)>	16.9	46.2	1.4
Completeness [%]	99.8	98.8	97.4
Redundancy	20.4	18.7	20.1
CC(1/2)	0.999	0.999	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	1.6 M magnesium sulfate and 100 mM MES pH 6.5