Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-01-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9768 |
| Spacegroup name | P 32 |
| Unit cell lengths | 135.242, 135.242, 262.353 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.480 - 4.120 |
| R-factor | 0.2528 |
| Rwork | 0.250 |
| R-free | 0.30300 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.950 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.480 | 4.250 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Number of reflections | 41317 | |
| <I/σ(I)> | 13.2 | 1.1 |
| Completeness [%] | 99.5 | 97.8 |
| Redundancy | 2.3 | 3.8 |
| CC(1/2) | 0.993 | 0.410 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.1 M Tris-HCl, pH 8.0, 4.5 M NaCl, 3% ethylene glycol |
