7UJ2
OspC Type B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-30 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.931, 44.085, 68.806 |
| Unit cell angles | 90.00, 103.83, 90.00 |
Refinement procedure
| Resolution | 36.797 - 1.503 |
| R-factor | 0.1475 |
| Rwork | 0.146 |
| R-free | 0.16720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ggq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.143 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.059 | 0.039 | 0.325 |
| Rmeas | 0.065 | 0.043 | 0.366 |
| Rpim | 0.028 | 0.018 | 0.164 |
| Total number of observations | 197208 | ||
| Number of reflections | 38181 | 2027 | 1922 |
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 95.8 | 97.5 | 95.6 |
| Redundancy | 5.2 | 5.7 | 4.6 |
| CC(1/2) | 0.998 | 0.932 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 100 mM sodium phosphate citrate pH 4.2, 41.9% PEG 600 |
