7UI2

The crystal structure of 15kDa Phlebotomus papatasi salivary protein Ppsp15.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	34.380, 54.610, 61.620
Unit cell angles	90.00, 89.97, 90.00

Refinement procedure

Resolution	40.870 - 1.190
R-factor	0.2133
Rwork	0.212
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ozd
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.260
High resolution limit [Å]	1.190	1.190
Rpim	0.066	0.484
Number of reflections	128818	9811
<I/σ(I)>	2.4	0.6
Completeness [%]	96.2	95.6
Redundancy	3.3
CC(1/2)	0.976	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	25% PEG 3350 0.2 M sodium chloride 0.1 M Tris-HCl pH 8.5