7UHE

Taf14 ET domain in complex with C-terminal tail of Taf2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2020-11-21
Detector	RDI CMOS_8M
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	47.889, 52.714, 121.507
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.450 - 1.660
R-factor	0.1948
Rwork	0.191
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6lqz
RMSD bond length	0.006
RMSD bond angle	0.865
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.690
High resolution limit [Å]	1.660	1.660
Rpim	0.027	0.108
Number of reflections	18278	734
<I/σ(I)>	48.5
Completeness [%]	98.2
Redundancy	9.6	2.5
CC(1/2)	0.992	0.943

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	296	2.4 M sodium malonate