7UH4

LXG-associated alpha-helical protein D2 (LapD2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-21
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.97926
Spacegroup name	P 31
Unit cell lengths	45.590, 45.590, 298.440
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.480 - 2.200
R-factor	0.2373
Rwork	0.233
R-free	0.26670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SAD model
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	xia2
Data scaling software	xia2
Phasing software	HKL-3000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.600	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.078	2.120
Number of reflections	34956	1569
<I/σ(I)>	12.9	0.8
Completeness [%]	99.3	90.6
Redundancy	11.1	6.6
CC(1/2)	0.999	0.474

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2M lithium sulfate, 0.1M Tris-HCl, pH 8.0, 30% PEG 4000