7UGS

Crystal structure of monomeric hyperfolder YFP (K206V mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	52.347, 66.659, 142.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.280 - 1.630
R-factor	0.1516
Rwork	0.150
R-free	0.18750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wj2
RMSD bond length	0.015
RMSD bond angle	1.599
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.280	1.688
High resolution limit [Å]	1.630	1.630
Rmerge	0.036	0.344
Rmeas	0.040	0.375
Rpim	0.016	0.148
Number of reflections	56130	3070
<I/σ(I)>	25.79	4.36
Completeness [%]	97.7	99.19
Redundancy	6.3	6.3
CC(1/2)	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291.15	0.1 M Tris-HCl pH 8.5, 25% PEG 3350, 25 mM magnesium chloride