7UG9

Crystal structure of RNase AM PHP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	40.289, 74.389, 40.079
Unit cell angles	90.00, 118.98, 90.00

Refinement procedure

Resolution	37.190 - 1.690
R-factor	0.1848
Rwork	0.182
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yb1
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.190	37.190	1.730
High resolution limit [Å]	1.690	7.560	1.690
Rmerge	0.113	0.114	0.582
Rmeas	0.139	0.140	0.728
Total number of observations	114177
Number of reflections	42453	513	2961
<I/σ(I)>	6.59	11.14	2.21
Completeness [%]	92.7	96.1	87.2
Redundancy	2.689	2.842	2.645
CC(1/2)	0.971	0.963	0.769

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20 % (w/v) PEG8000, 0.1 M Hepes pH 7.0