7UG2

Crystal structure of the coiled-coil domain of TRIM75

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	I 41 2 2
Unit cell lengths	43.070, 43.070, 134.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.985 - 2.052
Rwork	0.211
R-free	0.25620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7SI8
RMSD bond length	0.010
RMSD bond angle	1.806
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.042	0.248
Number of reflections	4039	594
<I/σ(I)>	14.1
Completeness [%]	96.3
Redundancy	3.2
CC(1/2)	0.998	0.913

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	298	0.1 M Acetate pH 4.6 20% Isopropanol 0.2 M Calcium chloride