7UFL

Crystal structure of chimeric omicron RBD (strain BA.2) complexed with chimeric mouse ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	80.048, 118.467, 110.845
Unit cell angles	90.00, 93.65, 90.00

Refinement procedure

Resolution	30.390 - 2.840
R-factor	0.2167
Rwork	0.214
R-free	0.27090
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6vw1
RMSD bond length	0.003
RMSD bond angle	0.603
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.852	3.200
High resolution limit [Å]	2.840	2.840
Rmerge	0.061	0.563
Number of reflections	26691	1335
<I/σ(I)>	10
Completeness [%]	54.5
Redundancy	3.7
CC(1/2)	0.999	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM Tris, pH 8.5, 18-22% PEG6000, 100 mM sodium chloride