7UFK

Crystal structure of chimeric omicron RBD (strain BA.2) complexed with human ACE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-23
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	80.397, 117.861, 113.018
Unit cell angles	90.00, 92.13, 90.00

Refinement procedure

Resolution	29.010 - 2.380
R-factor	0.194
Rwork	0.192
R-free	0.23530
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	6vw1
RMSD bond length	0.001
RMSD bond angle	0.388
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.600	2.710
High resolution limit [Å]	2.380	2.380
Rmerge	0.043
Number of reflections	43192	2160
<I/σ(I)>	17
Completeness [%]	51.4
Redundancy	4
CC(1/2)	0.999	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	100 mM Tris, pH 8.5, 18-22% PEG6000, 100 mM sodium chloride