7UE9
Structure of anti-C3d Fab(3d8b) in complex with C3d
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2020-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979257 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.372, 62.229, 85.547 |
| Unit cell angles | 90.00, 108.03, 90.00 |
Refinement procedure
| Resolution | 49.470 - 1.750 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ont |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.848 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.470 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 73836 | 11680 |
| <I/σ(I)> | 15.23 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.71 | |
| CC(1/2) | 0.998 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 15% PEG 10,000, 0.1 M bicine pH 7.6 |
